Search
Purdue School of Engineering and Technology

Purdue School of Engineering and Technology

Experimental Methods in Biomedical Engineering

BME 59500 / 3 Cr.

The course begins with the basic principles of hypothesis formulation and testing. Lectures rapidly progress toward the statistical design of experiments and proper selection of laboratory instrumentation, techniques and methodologies for testing a particular hypothesis, i.e. all experimental instrumentation and methodologies impart limits upon data interpretation relative to the specific biological questions understudy. Practical examples are derived from areas of neuroscience and cardiovascular research and involve a diverse range of instrumentation and methodologies including in vivo, in situ and in vitro electrophysiology (intra and extracellular recordings, care and use of animals, etc.), microscopy (optical, confocal, electron etc.) and fluorescent indicators (lipophilic dyes, antibody labeling, etc.) along with basic principles of noncontact in vivo imagining at the level of organ systems and cellular networks. Class time will also be devoted to development of experimental protocols that involve animal and human subjects, biosafety issues and the review processes for protocol submission.